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目的了解茂名市活禽市场环境禽流感病毒的污染状况,及时发现人禽流感发病潜在的危险因素。方法从3类场所每月采集20份外环境标本,采用荧光定量PCR检测A型、H5、H7、H9流感病毒核酸。结果 2013—2014年共采集并检测各类环境标本494份,其中A型阳性140份,阳性率28.34%。阳性标本中,H9亚型占57.86%;不同季节、场所和类型标本检测阳性率分别为冬春季节、农贸市场和清洗禽类的污水阳性率最高。结论茂名市活禽市场特别是农贸市场禽流感病毒污染广泛,家禽从业人员应做好个人防护,应加强禽流感的监测。
Objective To understand the pollution status of avian influenza virus in live poultry market in Maoming and find out the potential risk factors of human bird flu in time. Methods Twenty peripheral environmental samples were collected from 3 sites every month. Fluorescent quantitative PCR was used to detect influenza A, H5, H7 and H9 viruses. Results A total of 494 environmental samples were collected and tested during 2013-2014, of which 140 were positive for type A and the positive rate was 28.34%. In positive samples, H9 subtypes accounted for 57.86%. In different seasons, the positive rates of positive samples were in winter and spring, and the positive rates were highest in farmer’s markets and cleaning birds. Conclusion The live poultry market in Maoming City, especially the farmer’s market, has a wide range of bird flu infections. Poultry workers should be well protected and bird flu surveillance should be strengthened.