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用pH电位滴定法测定了苯丙氨酰亮氨酸(PL)与甘氨酸(Gly)、丙氨酸(Ala)、缬氨酸(Val)、亮氨酸(Leu)、异亮氨酸(Ile)、苯丙氨酸(Phe)在离子强度为0.1mol/L(NaNO_3),(25.0±0.1)℃时形成质子复合物的稳定常数,并对苯丙氨酰亮氨酸与六种氨基酸相互作用体系进行了量子化学和分子力学计算.从配体间弱相互作用的观点,讨论了二肽配体对氨基酸分子的识别.结果表明,二肽与氨基酸配体问的结合主要受到配体间静电、氢键作用的控制,同时受到范德华力和键间排斥能等弱相互作用的影响.
The contents of phenylalanyl leucine (PL) and glycine (Gly), alanine, val, leu and isoleucine were determined by pH potentiometric titration. ), The stability constant of phenylalanine (Phe) proton complex was formed when the ionic strength was 0.1mol / L (NaNO_3) and (25.0 ± 0.1) ℃, and the interaction between phenylalanyl leucine and six amino acids The interaction between dipeptide and amino acid ligand is mainly affected by the interaction between dipeptide ligand and amino acid ligand.According to the weak interaction between ligands, Electrostatic, hydrogen bond control, at the same time by the van der Waals force and the bond between the repulsive energy and other weak interactions.