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目的测定分析福建省2012-2014年登革病毒E基因序列,探讨病毒传播来源及基因型。方法收集福建省2012-2014年登革热患者血清样本33份,RT-PCR法扩增登革病毒E基因,测定基因序列并绘制系统发育树,结合流行病学资料分析。结果福建省2012-2014年登革热主要来源仍为输入性,可能来源于东南亚国家,以DENV-1和DENV-2基因型为主。基于E基因系统进化分析表明,福建省2012-2014年DENV-1属于GenetypeⅠ、GenetypeⅣ和GenetypeⅤ;DENV-2基因型均为Cosmopolitan(混合型)。结论福建省2012-2014年登革病例仍以输入为主,存在输入引起本地感染的风险,应加强出入境人员的监测工作。
Objective To analyze and analyze the sequence of dengue virus E gene from 2012 to 2014 in Fujian Province and explore the origin and genotype of virus transmission. Methods A total of 33 serum samples from dengue fever patients in Fujian province from 2012 to 2014 were collected. The dengue virus E gene was amplified by RT-PCR. The gene sequence was determined and phylogenetic tree was drawn. The epidemiological data were analyzed. Results The main source of Dengue Fever in Fujian Province during 2012-2014 was still imported, probably from Southeast Asian countries, with DENV-1 and DENV-2 genotypes as the main sources. Phylogenetic analysis based on E gene indicated that DENV-1 belonged to GenetypeⅠ, GenetypeⅣ and GenetypeⅤ from 2012 to 2014 in Fujian province, and Cosmopolitan (mixed type) in DENV-2 genotype. Conclusions The cases of dengue in Fujian Province during 2012-2014 are still dominated by input. There is a risk that the input may cause local infection, and the surveillance of entry-exit personnel should be strengthened.