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目的:探讨Ag85B在诱发抗黑素瘤免疫应答中的作用,以及其用于肿瘤免疫治疗的可行性。方法:经背部皮下接种黑色素瘤细胞株B16建立C57BL/6小鼠荷瘤模型,并分为对照1、2组和实验组。于荷瘤模型建立后,对照1、2组经背部皮下分别接种B16细胞和B16/pcDNA3细胞,实验组背部皮下接种B16/pcDNA3-Ag85B细胞。分别观测3组小鼠肿瘤的体积、生存时间和血清中的IFN-γ及IL-4的含量。研究Ag85B在诱导抗肿瘤免疫应答中的作用。结果:从13d到23d,对照1组和对照2组荷瘤小鼠,肿瘤的平均体积分别从1.1058cm3和0.9123cm3增长到7.5983cm3和5.8746cm3;而实验组荷瘤小鼠,肿瘤的平均体积由0.5158cm3增长到1.5080cm3。对照1组和对照2组荷瘤小鼠的平均生存时间分别为24.1d和24.7d,而实验组荷瘤小鼠的平均生存时间为27.8d。13d内,两对照组和实验组小鼠血清IFN-γ的含量均有所上升(对照1组、对照2组和实验组分别上升至26.3ng/L、23.0ng/L和25.2ng/L),随后两对照组小鼠血清IFN-γ的含量均呈下降趋势(对照1组和对照2组分别下降至19.3ng/L和18.3ng/L),而实验组小鼠血清IFN-γ的含量则呈上升趋势(上升至46.5ng/L)。13d内,两对照组和实验组小鼠血清IL-4的含量均有轻微升高,随后逐渐下降,但3组小鼠血清IL-4含量的变化差异不明显。结论:接种B16/pcDNA3-Ag85B细胞的实验组小鼠血清中IFN-γ的含量明显增高,并可抑制荷瘤小鼠体内肿瘤的生长,延长荷瘤小鼠生存的时间。
Objective: To investigate the role of Ag85B in inducing anti-melanoma immune response and its feasibility for tumor immunotherapy. Methods: C57BL / 6 mice were inoculated with B16 melanoma cell line subcutaneously in the back and divided into control group 1, group 2 and experimental group. After the establishment of the tumor model, B16 and B16 / pcDNA3 cells were inoculated subcutaneously into the control group and the B16 / pcDNA3 cells subcutaneously in the back of the mice. The back of the experimental group was inoculated with B16 / pcDNA3-Ag85B cells. The tumor volume, survival time and serum levels of IFN-γ and IL-4 in 3 groups of mice were observed respectively. To study the role of Ag85B in inducing anti-tumor immune responses. Results: From the 13th day to the 23rd day, the average volume of the tumor in the control group 1 and the control group 2 increased from 1.1058cm3 and 0.9123cm3 to 7.5983cm3 and 5.8746cm3, respectively. However, in the experimental group, the mean volume of the tumor Increased from 0.5158cm3 to 1.5080cm3. The average survival time of tumor-bearing mice in control group 1 and control group 2 was 24.1d and 24.7d, respectively. The average survival time of mice in experimental group was 27.8d. Within 13 days, the levels of IFN-γ in two control and experimental groups increased (control group 1, control group 2 and experimental group increased to 26.3ng / L, 23.0ng / L and 25.2ng / L respectively) , While the levels of IFN-γ in the two control groups showed a decreasing trend (19.3ng / L and 18.3ng / L in control group 1 and control group 2, respectively), while the level of serum IFN-γ in experimental group The trend is up (up to 46.5ng / L). Within 13 days, the levels of IL-4 in both control and experimental groups increased slightly, and then gradually decreased. However, there was no significant difference in serum IL-4 level among the three groups. CONCLUSION: The level of IFN-γ in sera of mice inoculated with B16 / pcDNA3-Ag85B cells is significantly higher than that of the control group, which can inhibit the growth of tumor in tumor-bearing mice and prolong the survival time of tumor-bearing mice.