造血干细胞体外培养时,除必要的培养液外,需要有滋养细胞层参与。参与粒细胞团形成的物质称为造血灶刺激因子(简写CSF)。在实验研究工作中,一般采用的造血灶刺激因子有胚胎细胞条件培养液、注射伤寒副伤寒甲菌疫苗后的小鼠肺脏培养液、大鼠注射内毒素后的血清,或者用肺组织块作双层培养。我们发现肌组织可产生丰富的造血灶刺激因子,可以实际应用。 Hematopoietic stem cells cultured in vitro, in addition to the necessary culture medium, the need for trophoblast layer involvement. Materials involved in the formation of granulocytes are called hematopoietic stimulators (abbreviated CSF). In the experimental research work, the general use of hematopoietic stimulating factor embryo cell conditioned medium, injection of Salmonella typhimurium vaccine lung murine culture, rat after injection of endotoxin serum, or lung tissue block Bilayer culture. We found that muscle tissue can produce a wealth of hematopoietic stimulating factors that can be used in practice.