论文部分内容阅读
目的:探讨Lgr5基因在新疆维、汉族直肠癌组织和癌旁组织中的表达及其与临床病理因素的关系。方法:收集我院2014年1月至2016年3月期间住院的Ⅱ、Ⅲ期准备行术前放化疗的直肠癌患者肠镜活检组织,癌组织及对应癌旁组织各94例,其中汉族50例,维吾尔族44例,应用免疫组织化学(SP)法和实时荧光(qRT-PCR)法分别检测直肠癌组织和癌旁组织中Lgr5蛋白和mRNA的表达。结果:直肠癌组织中Lgr5蛋白的阳性表达率为85.1%,高于癌旁组织的24.5%,差异有统计学意义(P<0.05);Lgr5 mRNA表达水平在癌组织中(9.77±7.04)高于癌旁组织(3.25±2.89),差异有统计学意义(P<0.05);维吾尔族、汉族Lgr5蛋白和mRNA表达在癌组织和癌旁组织中均无统计学差异(P>0.05)。Lgr5蛋白表达与TNM分期和浸润深度有关,Lgr5 mRNA表达与组织分化程度和淋巴结转移有关。结论:Lgr5基因表达与直肠癌的发生、发展有相关性;Lgr5基因表达在维、汉不同民族之间无显著性差异。
Objective: To investigate the expression of Lgr5 gene in rectal cancer tissues and paracancerous tissues in Xinjiang Uygur and Han nationality and its relationship with clinicopathological factors. Methods: A total of 94 cases of rectal cancer patients with stage Ⅱ and Ⅲ admitted to our hospital from January 2014 to March 2016 were enrolled in this study. There were 94 cases of colon cancer, In 44 cases of Uygur, the expression of Lgr5 protein and mRNA in rectal cancer tissues and adjacent tissues were detected by immunohistochemistry (SP) and real-time fluorescence (qRT-PCR) Results: The positive rate of Lgr5 protein in rectal cancer tissues was 85.1%, which was higher than that in paracancerous tissues (P <0.05). The expression of Lgr5 mRNA in cancer tissues was (9.77 ± 7.04) high (3.25 ± 2.89) (P <0.05). There was no significant difference in the expression of Lgr5 protein and mRNA between Uighur and Han nationality (P> 0.05). Lgr5 protein expression and TNM stage and depth of invasion, Lgr5 mRNA expression and tissue differentiation and lymph node metastasis. Conclusion: The expression of Lgr5 gene is correlated with the occurrence and development of rectal cancer. The expression of Lgr5 gene has no significant difference between Victoria and Han ethnic groups.