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目的 :探讨一氧化氮合酶 (NOS)神经在兔不全梗阻性膀胱中的变化及意义。 方法 :采用还原型烟酰胺腺嘌呤二核苷酸磷酸 (NADPH)免疫组化方法 ,对 10只新西兰白兔 (成年雄性 )不全梗阻性膀胱 (自制兔不全梗阻性膀胱 ,梗阻时间为 6周 ,作为实验组 )及 10只无梗阻性膀胱同龄新西兰白兔 (对照组 )膀胱体及膀胱颈部组织进行研究。 结果 :实验组兔膀胱体、膀胱颈部粘膜及膀胱体平滑肌NOS神经均明显减少 (膀胱体粘膜为P <0 .0 5 ,膀胱颈粘膜为P <0 .0 1,膀胱体平滑肌为P <0 .0 5 ) ;膀胱颈部平滑肌NOS神经无显著变化。 结论 :一氧化氮对平滑肌具有松弛作用 ,NOS神经的减少对兔不全梗阻性膀胱的结构和功能变化具有一定作用。
Objective: To investigate the changes and significance of nitric oxide synthase (NOS) nerve in incomplete obstructive bladder in rabbits. Methods: 10 New Zealand white rabbits (adult male) with incomplete obstructive bladder (self-made rabbits with incomplete obstructive bladder, obstruction time of 6 weeks, As the experimental group) and 10 non-obstructive bladder-aged New Zealand white rabbits (control group) bladder and bladder neck tissue were studied. Results: NOS nerves of bladder, bladder neck mucosa and smooth muscle of bladder in experimental group were significantly decreased (P <0.05 for bladder mucosa, P <0.01 for bladder neck mucosa, P < 0 .0 5); bladder neck smooth muscle NOS no significant changes in nerve. CONCLUSION: Nitric oxide can relax the smooth muscle. The decrease of NOS nerve may play a role in the structural and functional changes of incomplete obstructive bladder in rabbits.