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目的：探讨胃泌素类似物五肽胃泌素（ＰＧ）及胃泌素受体拮抗剂丙谷胺（ＰＧＬ）对体外培养的人结肠癌细胞株ＨＴ２９生长的影响。方法：采用ＭＴＴ法检测细胞增殖情况用流式细胞术（ＦＣＭ）分析细胞周期变化。结果：高浓度的ＰＧ（１０－５～１０－７Ｍ）能明显促进ＨＴ２９细胞的增殖（Ｐ＜００５），低浓度的ＰＧ（１０－８～１０－１０Ｍ）对ＨＴ２９细胞无明显促增殖作用（Ｐ＞００５）。ＰＧＬ能抑制ＰＧ对ＨＴ２９细胞的刺激作用，并对ＨＴ２９细胞的增殖有直接抑制作用（Ｐ＜００５）。细胞周期分析显示ＰＧ能缩短细胞Ｇ３／Ｇ１期，ＰＧＬ则延长细胞Ｇ０／Ｇ１期（Ｐ＜００５）。结论：胃泌素对体外培养的ＨＴ２９细胞的生长具有调节作用，胃泌素受体拮抗剂可能在结肠癌的治疗中发挥重要作用。
Objective: To investigate the effect of gastrin analog pentagastrin (PG) and gastrin receptor antagonist proglumide (PGL) on the growth of human colon cancer cell line HT29 in vitro. Methods: Cell proliferation was measured by MTT assay. Cell cycle changes were analyzed by flow cytometry (FCM). Results: High concentration of PG (10-5～10-7M) can significantly promote the proliferation of HT29 cells (P<0.05). Low concentration of PG (10-8～10-10M) does not significantly promote proliferation of HT29 cells. Role (P>005). PGL can inhibit the stimulatory effect of PG on HT29 cells and directly inhibit the proliferation of HT29 cells (P<005). Cell cycle analysis showed that PG could shorten the G3/G1 phase, and PGL prolonged G0/G1 phase (P<005). Conclusion: Gastrin can regulate the growth of HT29 cells cultured in vitro. Gastrin receptor antagonists may play an important role in the treatment of colon cancer.