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用细粒棘球绦虫成虫虫体抗原及其免疫家兔血清ＩｇＧ建立了用于检测家犬粪抗原诊断细粒棘球绦虫成虫感染的双抗体夹心ＥＬＩＳＡ方法。对用离子交换层析、ＳＰＡ亲和层析、抗兔ＩｇＧ抗体亲和层析和细粒棘球绦虫成虫虫体抗原亲和层析等四种方法纯化的抗体进行了比较，以抗原亲和层析法纯化的抗体免疫活性最高，用双抗体夹心ＥＬＩＳＡ检测抗原的终点在标本缓冲液中为２．５ｎｇ／ｍｌ，在健康家犬粪便悬液的上清中为５ｎｇ／ｍｌ。检测１１只人工感染细粒棘球绦虫成虫的家犬粪便标本，粪抗原阳性率达１００％。
A double antibody sandwich ELISA method was developed for the detection of adult dog fecal antigen for diagnosis of adult worm particles of Echinococcus granulosus, using the adult worm antigen of Echinococcus granulosus and its immunized rabbit serum IgG. Antibodies purified by four methods of ion exchange chromatography, SPA affinity chromatography, anti-rabbit IgG affinity chromatography and Echinococcus granulosus adult worm antigen affinity chromatography were compared. Antigen affinity The purified antibody by chromatography was the most immunocompetent. The endpoint of the antigen detected by the double antibody sandwich ELISA was 2.5 ng / ml in the specimen buffer and 5 ng / ml in the supernatant of the healthy dog feces suspension. Detection of 11 artificial infection Echinococcus granulosus adults stool specimens of feces, fecal antigen-positive rate of 100%.