报道了7个金黄色葡萄球菌核酸酶(SNase)C-末端缺失突变体在E. coli细胞中的高效表达,其表达量可占细胞总蛋白量的16.90％～57.7％,即使仅由52个残基组成的小肽段,也能在细胞中稳定积累,然而,SNase的C-末端缺失明显地影响到肽段的分泌表达水平乃至越膜分泌。实验指出,7个肽段都能同抗SNase多克隆抗体呈特异性免疫反应,较长的肽段仍保留着不同程度的SNase的催化活性。 Reported the high expression of seven C-terminal deletion mutants of S. aureus nuclease (SNase) in E. coli cells, which accounted for 16.90% ~ 57.7% of the total cellular protein, even though only 52 However, the deletion of the C-terminal of SNase significantly affects the secretion and secretion of the peptide. Experiments show that all seven peptides can react specifically with anti-SNase polyclonal antibodies. Longer peptides still retain different degrees of catalytic activity of SNase.