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目的：建立骨碎补药材的HPLC指纹图谱提供骨碎补质量控制可靠方法。方法采用HPLC法色谱柱为DianosilTM C18柱（200mm×4.6mm,5um）：流动相为乙腈-0.4%冰醋酸水溶液，梯度洗脱，柱温25℃，流速为1.0ml·min-1，检测波长254nm。结果初步建立了骨碎补药材的 HPLC指纹图谱，标定了8个共有峰，测定10批骨碎补正品中，3种成分含量，抽皮苷0.49~0.76%，新北美圣草苷0.49~0.63%，E-4-0-β-D葡萄糖酰咖啡酸0.11%~0.36%，鉴别了真伪骨碎补。结论利用 HPLC指纹图谱可从定性和定量两方面控制骨碎补药材的内在质量，方法稳定、简便、快捷。“,”Objective: To establish HPLC fingerprint of Rhizoma Drynariae for its reliable quality control. Methods: HPLC was adopted with DianosilTM C18 column (200mm×4.6mm, 5um) as chromatographic column. Mobile phase was acetonitrile-aqueous solution of glaciallacetic acid with 0.4%. Gradient elution was performed with column temperature 25℃, flow rate 1.0 ml.min-1, detection wavelength 254nm. Results: HPLC fingerprint of Rhizoma Drynariae was initially established with 8 common peaks calibrated, which was used to identify between true Rhizoma Drynariae and false products in 10 batches detected quality products by contents of 3 components:naringin 0.49-0.76%, neoeriocitrin0.49-0.63%. E-4-0-β-D glucopyranosyllcaf eic acid 0.11~0.36%. Conclusion: HPLC fingerprint could be used to controllthe inner quality of Rhizoma Drynariae from two aspects of qualification and quantitation, which was stable, simple and fast.