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目的:研究肝郁脾虚证胃溃疡大鼠经痛泻要方治疗后胃窦组织蛋白质组学的变化,探讨肝郁脾虚证胃溃疡发病及痛泻要方可能的作用机制。方法:建立阿司匹林诱发大鼠胃溃疡及多种不同应激方法进行情志刺激引起的大鼠肝郁脾虚证模型,通过痛泻要方动物体内给药,研究其对于大鼠一般体征、溃疡指数、差异蛋白表达的影响。采用黏膜损伤评分法研究痛泻要方对大鼠溃疡指数的影响;采用大鼠胃窦组织样本i TRAQ蛋白质组学检测方法,探寻差异蛋白表达。结果:痛泻要方能改善胃溃疡大鼠一般体征,明显减轻胃溃疡大鼠的溃疡指数并明显减轻胃溃疡大鼠的胃液酸度,差异蛋白筛选、Gene ontology分析、差异蛋白pathway分析及基因网络分析表明,痛泻要方对肝郁脾虚证胃溃疡胃窦部差异蛋白及差异基因的表达水平有明显的修复作用,痛泻要方对肝郁脾虚证大鼠有机物代谢、大分子代谢、初级代谢、细胞代谢过程、单有机体代谢过程有明显的调控作用,并对Kit受体信号通路、蛋白酶体降解、细胞因子及炎症反应、IL-5信号通路、Omega脂肪酸氧化、MAPK信号通路、mRNA加工、补体活化途径、炎症反应通路、Statin通路、IL-3信号通路、凝血通路、条纹肌收缩、TNF-α及NF-κB信号通路、EGFR1信号通路、胰岛素信号通路、补体和凝血级联反应通路、胞浆核糖体蛋白通路等炎症通路、免疫调节通路、细胞增殖及凋亡通路及分子具有广泛的调节作用。结论:痛泻要方对阿司匹林及多种不同应激方法进行情志刺激引起的肝郁脾虚证胃溃疡模型具有明显的治疗作用,肝郁脾虚证胃溃疡发病会导致胃窦组织蛋白质组学的显著变化,而痛泻要方对差异蛋白及信号通路具有显著的修复调节作用。
Objective: To study the changes of proteomics of gastric antrum after treatment of Tongyu Xieya Decoction in rats with liver-qi and spleen-deficiency syndrome and to explore the possible mechanism of the pathogenesis of gastric ulcer and the prescription of diarrhea in rats with liver-qi and spleen-deficiency syndrome. Methods: To establish aspirin - induced gastric ulcer and a variety of different stress emotional stimulation induced rat model of liver spleen deficiency syndrome, by diarrhea Yao Fang animal in vivo administration to study the general signs of rats, ulcer index , The impact of differential protein expression. Mucosal damage score was used to study the effect of Tongxie Yaofang on ulcer index in rats. The i TRAQ proteomics method was used to detect the differential protein expression in gastric antrum. Results: Tong Xie Yao Fang can improve the general signs of gastric ulcer rats, significantly reduce the ulcer index of gastric ulcer rats and significantly reduce gastric ulcer gastric acidity, differential protein screening, Gene ontology analysis, differential protein pathway analysis and gene network The analysis shows that Tongxieyaofang has significant repair effect on the expression of differentially expressed proteins and differential genes in gastric antrum of patients with liver-qi and spleen-deficiency syndrome. Tongxieyaofang can inhibit the metabolism of organic compounds, macromolecule metabolism, Metabolism, cellular metabolism and metabolic processes of single organism have obvious regulatory effects on Kit receptor signaling pathway, proteasomal degradation, cytokines and inflammatory responses, IL-5 signaling pathway, Omega fatty acid oxidation, MAPK signaling pathway, mRNA processing , Complement activation pathway, inflammatory response pathway, Statin pathway, IL-3 signaling pathway, coagulation pathway, striated muscle contraction, TNF-alpha and NF-κB signaling pathway, EGFR1 signaling pathway, insulin signaling pathway, complement and coagulation cascade pathway , The cytoplasmic ribosomal protein pathway and other inflammatory pathways, immunoregulatory pathways, cell proliferation and apoptosis pathways and molecules have a wide range The regulatory role. Conclusion: Tong Xie Yao Fang for aspirin and a variety of different stress induced emotional stimulation caused by liver stagnation and spleen deficiency gastric ulcer model has obvious therapeutic effect, liver depression and spleen deficiency gastric ulcer pathogenesis can lead to antral proteomic Significant changes, and Tong Xie Yao Fang on differential proteins and signaling pathway has a significant role in the repair regulation.