论文部分内容阅读
目的:比较不同萃取剂及提取方式对房水中维生素A棕榈酸酯(VAP)提取率的影响,并用HPIC法测定兔眼房水中VAP浓度,提供其药动学实验基础。方法:分别比较二氯甲烷、氯仿、正己烷及乙醚萃取兔房水中VAP的提取率及不同处理提取方式对提取率的影响。以甲醇沉淀兔眼房水中的蛋白质后进行HPLC测定,流动相为甲醇,检测波长为327 nm,流速l ml·min~(-1),柱温35℃。结果:二氯甲烷、氯仿萃取法对VAP提取率较低;而乙醚与标准应用液及房水互溶达不到萃取目的;正己烷萃取法制备的房水样品VAP提取率较高。兔眼房水中VAP浓度在0.047～4.080μg·ml~(-1)范围内呈良好的线性关系,方法学回收率99.0%,绝对回收率96.1%,日内和日间RSD小于10%。结论:本方法具有灵敏、准确、快速等特点,可应用于眼房水VAP的药动学研究。
OBJECTIVE: To compare the effects of different extractants and extraction methods on the extraction rate of vitamin A palmitate (VAP) in aqueous humor and to determine the concentration of VAP in rabbit aqueous humor by HPIC method and provide the basis of its pharmacokinetics. Methods: The extraction rates of VAP in rabbit aqueous humor from dichloromethane, chloroform, n-hexane and ether were compared respectively, and the effects of different extraction methods on the extraction rate were compared. After precipitation of protein in rabbit aqueous humor by methanol, the mobile phase was methanol. The detection wavelength was 327 nm, the flow rate was 1 ml · min ~ (-1) and the column temperature was 35 ℃. Results: The extraction rate of VAP by dichloromethane and chloroform was lower than that of standard solution and aqueous solution. The extraction rate of VAP from aqueous sample prepared by n-hexane extraction was higher. Rabbit aqueous humor in the range of 0.047 ~ 4.080μg · ml -1 VAP concentration showed a good linear relationship, the methodological recovery rate of 99.0%, the absolute recovery rate of 96.1%, intraday and interday RSD less than 10%. Conclusion: The method is sensitive, accurate and rapid and can be applied to the pharmacokinetics study of VAP in aqueous humor.