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背景:过氧化脂质、单胺氧化酶、超氧化物歧化酶是测定体内自由基清除能力的重要生化药理指标。过氧化脂质还可作为机体衰老氧化的一个定量指标。目的:通过测定衰老模型大鼠血清中单胺氧化酶、过氧化脂质及超氧化物歧化酶活性,验证太白洋参的抗自由基作用。设计:随机对照实验。单位:陕西中医学院药学系。材料:实验于2002-03/04在陕西中医学院药理实验基地完成。选择健康SD青年、老年大鼠各40只,太白洋参药材粉碎用水提取,并将滤液浓缩至1.5g/mL。人参用水煎煮提取并浓缩至0.3g/mL。方法:青年大鼠和老年大鼠随机分为8组:青年正常对照组、青年人参水煎液组、青年太白洋参大剂量组和青年太白洋参小剂量组。老年正常对照组、老年人参水煎液组、老年太白洋参大剂量组和老年太白洋参小剂量组,每组10只。适应环境7d后,正常对照组每日用20g/kg蒸馏水灌胃,人参水煎液组用人参水煎液按4g/(kg·d)灌胃,太白洋参大剂量组和小剂量组分别用20g/(kg·d),10g/(kg·d)灌胃,35d后于麻醉状态下断头取血处死。收集血清,离心后采用硫化巴比妥酸法测定丙二醛浓度、采用紫外吸收法测定单胺氧化酶活性、采用黄嘌呤氧化酶法测定超氧化物歧化酶活性。主要观察指标:青年和老年大鼠血清丙二醛浓度、单胺氧化酶、超氧化物歧化酶活性。结果:80只大鼠均进入结果分析。①人参水煎液组和太白洋参大、小剂量组的丙二醛含量和单胺氧化酶活性均低于正常对照组(P<0.05～0.01),超氧化物歧化酶活性高于正常对照组(P<0.05～0.01)。②老年太白洋参小剂量组大鼠血清中丙二醛含量和单胺氧化酶活性高于青年太白洋参小剂量组大鼠[(8.23±1.73),(6.73±1.69)μmol/L;(41.7±15.9),(40.9±14.6)nkat/L,P<0.05],而老年太白洋参大、小剂量组超氧化物歧化酶活性低于青年太白洋参大、小剂量大鼠[(386.75±26.71),(403.49±21.58)NU/mL;(382.13±20.07),(389.69±24.45)NU/mL,P<0.05]。结论:太白洋参具有明显的抑制单胺氧化酶作用及减少过氧化脂质的功能,且能增强超氧化物歧化酶的活性,说明太白洋参具有抗自由基损伤能力,能够延缓衰老。
BACKGROUND: Lipid peroxide, monoamine oxidase, and superoxide dismutase are important biochemical pharmacological parameters for the determination of free radical scavenging activity in vivo. Lipid peroxide can also serve as a quantitative indicator of aging oxidation in the body. Objective: To verify the anti-free radical effects of Taibai ginseng by measuring the activities of monoamine oxidase, lipid peroxide and superoxide dismutase in serum of aging rats. Design: Randomized controlled trials. Unit: Department of Pharmacy, Shaanxi College of Traditional Chinese Medicine. MATERIALS: The experiment was completed at the Pharmacological Experimental Base of Shaanxi College of Traditional Chinese Medicine from March to April 2002. 40 healthy SD youths and 40 old ageing rats were selected. The Chinese medicinal materials of Taibai Ginseng were crushed and extracted with water, and the filtrate was concentrated to 1.5 g/mL. Ginseng was extracted with water and concentrated to 0.3 g/mL. Methods: Young rats and aged rats were randomly divided into 8 groups: young normal control group, young ginseng decoction group, young Taibai ginseng high dose group and young Taibai ginseng low dose group. Senile normal control group, elderly decoction decoction group, old Taibai ginseng high-dose group and old Taibai ginseng low-dose group, 10 in each group. After 7 days of adaptation to the environment, the normal control group was treated with 20 g/kg distilled water daily. The ginseng decoction group was given intragastrically with ginseng decoction at 4g/(kg·d), and the high-dose and low-dose groups of Taibai ginseng were administered separately. 20 g/(kg·d) and 10 g/(kg·d) were intragastrically administered. After 35 days, they were sacrificed by taking blood from anesthesia. The serum was collected and centrifuged. The concentration of malondialdehyde was measured by barbituric acid sulphide method, the monoamine oxidase activity was measured by ultraviolet absorption method, and the superoxide dismutase activity was measured by xanthine oxidase method. MAIN OUTCOME MEASURES: Serum MDA concentrations, monoamine oxidase, and superoxide dismutase activities in young and aged rats. RESULTS: All 80 rats entered the results analysis. 1The content of MDA and the activity of monoamine oxidase in Ginseng Decoction group and Taibai Ginseng group were lower than normal control group (P<0.05-0.01), and the activity of superoxide dismutase was higher than normal control group (P). <0.05-0.01). 2The content of malondialdehyde and monoamine oxidase activity in the serum of the old Taibai Ginseng low-dose group was higher than that of the Young Taibai Ginseng low-dose group [(8.23±1.73), (6.73±1.69) μmol/L; (41.7±15.9) (40.9±14.6) nkat/L, P<0.05], while the activity of superoxide dismutase in the old and small doses of Taibai Ginseng was lower than that in young large and small doses of Taibai Ginseng [(386.75±26.71)]. (403.49±21.58) NU/mL; (382.13±20.07), (389.69±24.45) NU/mL, P<0.05]. Conclusion: Taibai ginseng has obvious functions of inhibiting monoamine oxidase and reducing lipid peroxidation, and can enhance the activity of superoxide dismutase, indicating that Taibai ginseng has anti-free radical damage ability and can delay aging.