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LT酶活性浓度测定的仪器常数K值通常采用计算方法 (K =Tυε d υ 10 6)求得 . NADH的理论值为 6 2 2 0。本文运用ALT酶促反应的产物丙酮酸和己糖激酶 (HK)的底物葡萄糖为标准 ,按终点法测定K值 (K =CstAst-Ab)。理论上计算方法求得K值应该与终点法测定的K值相同 (Tυε d υ 10 6=CstAst-Ab)。实验提示同一台生化仪采用丙酮酸为标准和葡萄糖为标准按终点法测定的K值一致。但与计算方法测定的K值存在着差异。建议各种酶活性浓度测定选择有效的产物或底物作为校正标准 ,达到酶活性浓度测定的一致性。
The value of the instrumental constant K for the determination of LT enzyme activity concentration is usually obtained by the calculation method (K=Tυε d υ 10 6 ). The theoretical value of NADH is 6 2 2 0 . In this paper, the substrate glucose of pyruvate and hexokinase (HK), the product of the ALT enzymatic reaction, was used as the standard and the K value was determined by the endpoint method (K = CstAst-Ab). Theoretically, the K value obtained by the calculation method should be the same as the K value determined by the endpoint method (Tυε d υ 106 = CstAst-Ab). The experiment indicated that the same biochemical analyzer used pyruvate as the standard and glucose as the standard. The K value determined by the endpoint method was the same. However, there is a difference in the K value determined by the calculation method. It is recommended that various enzyme activity concentrations be determined by selecting an effective product or substrate as the calibration standard to achieve consistency in the determination of enzyme activity concentration.