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采用菌丝生长速率法,测定了可引起杨树炭疽病的59株胶孢炭疽菌和4株炭疽菌对多菌灵、苯醚甲环唑、戊唑醇和三唑酮4种杀菌剂的敏感性。结果表明:59株胶孢炭疽菌对多菌灵、苯醚甲环唑、戊唑醇和三唑酮的EC_(50)值范围分别在0.037 1~0.130 1、0.102 5~1.680、0.069 1~1.917及3.053~38.59μg/m L之间,平均EC_(50)值分别为(0.066 4±0.013 1)、(0.374 1±0.254 8)、(0.681 2±0.442 1)和(19.82±6.200)μg/m L。胶孢炭疽菌对多菌灵的敏感性频率分布呈连续单峰曲线,表明尚未出现敏感性下降的群体;而对苯醚甲环唑和戊唑醇的敏感性频率分布呈现双峰,表明已出现敏感性下降的群体。4株炭疽菌对多菌灵、苯醚甲环唑、戊唑醇和三唑酮的最小EC_(50)值和最大EC_(50)值分别相差1.33、13.19、13.66和2.14倍,其中菌株Ca-4对苯醚甲环唑和戊唑醇的EC_(50)值均大于4μg/m L。不同寄主来源的胶孢炭疽菌对同种杀菌剂的敏感性之间无显著差异(P>0.05)。Spearman’s秩相关分析表明,胶孢炭疽菌对苯醚甲环唑和戊唑醇的敏感性之间呈显著正相关性(ρ=0.665 5,P<0.000 1),对戊唑醇和三唑酮的敏感性之间也存在一定相关性(ρ=0.489 6,P<0.000 1),其余杀菌剂之间则均无相关性(P>0.05)。研究结果对合理使用杀菌剂防治杨树炭疽病具有借鉴意义和参考价值。
The mycelium growth rate method was used to determine the susceptibility of 59 strains of Colletotrichum gloeosporioides and 4 strains of anthracnose to the fungicides carbendazim, difenoconazole, tebuconazole and triadimefon, Sex. The results showed that EC 59 values of carbendazim, difenoconazole, tebuconazole and triadimefon were in the range of 0.037 1 ~ 0.130, 0.102 5 ~ 1.680, 0.069 1 ~ 1.917 And 3.053 ~ 38.59μg / m L, the average EC50 values were (0.066 4 ± 0.013 1), (0.374 1 ± 0.254 8), (0.681 2 ± 0.442 1) and (19.82 ± 6.200) μg / m L. The frequency distribution of anthracnose isolates to carbendazim showed a continuous unimodal curve, indicating that no sensitivities had been found. The frequency distribution of difenoconazole and tebuconazole showed a double peak indicating that A population with decreased sensitivity appears. The four strains of anthracnose had a difference of 1.33, 13.19, 13.66 and 2.14 times of the minimum EC 50 value and the maximum EC 50 value of carbendazim, difenoconazole, tebuconazole and triadimefon, respectively. The strain Ca- The EC 50 values of 4-difenoconazole and tebuconazole were higher than 4μg / m L. There was no significant difference (P> 0.05) between the isolates of Colletotrichum gloeosporioides and different fungicides. Spearman’s rank correlation analysis showed that there was a significant positive correlation between the susceptibilities of Tetracenomycosis to difenoconazole and tebuconazole (ρ = 0.665 5, P <0.000 1) There was also a certain correlation between the sensitivity (ρ = 0.489 6, P <0.000 1), but no correlation was found between the other fungicides (P> 0.05). The research results have reference significance and reference value for the rational use of bactericide to prevent and cure poplar anthracnose.