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Trager蜡烛缸培养恶性疟原虫的方法,每天需换培养液一次。现利用蜡烛缸方法,通过降低虫血悬液中的红细胞压积和起始原虫率来延长更换营养液的时间,获得了较满意的培养结果。 材料和方法 一、材料:以已建立体外培养并已适应兔血清培养的FCC-1/HN株恶性疟原虫作虫源。以RPMI 1640和HEPES缓冲剂参照Trager(1978)和Jensen(1979)方法配制成培养液。兔血清含量为15％。
Trager candle barrel method of culturing P. falciparum, change the culture fluid once a day. Now using the candle cylinder method, by reducing the hematocele hematocrit and the initial protozoan rate to extend the replacement of nutrient solution time, obtained more satisfactory culture results. MATERIALS AND METHODS I. Materials: Plasmodium falciparum from strain FCC-1 / HN, which had been cultured in vitro and had been adapted to rabbit serum, was used as an insect source. Media was prepared as described in Trager (1978) and Jensen (1979) using RPMI 1640 and HEPES buffer. Rabbit serum content of 15%.