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目的:探讨药用植物湖北麦冬花药愈伤组织诱导和植株再生条件。方法:以湖北麦冬花药为外植体,采用MS培养基,附加不同的植物激素进行实验。常规压片法结合显微镜进行再生植株染色体的计数分析。结果:MS+2,4-D 1.0 mg.L-1+KT 2.0 mg.L-1诱导愈伤组织效果最好,愈伤组织诱导率可达41.07%。MS+6-BA 1.5~2.0 mg.L-1+NAA 0.1~0.3mg.L-1适于不定芽的诱导,不定芽转入附加NAA 0.1~0.3 mg.L-1的1/2 MS的生根培养基上,生根后获得完整的再生植株,再生植株为体细胞起源。同时,讨论了4℃低温预处理对愈伤组织诱导的影响。结论:建立了湖北麦冬花药体细胞组织培养体系和快速繁殖途径。
Objective: To investigate the induction and plant regeneration conditions of the medicinal plant Radix Ophiopogon japonicus callus. Methods: Ophiopogon japonicus was used as explants and MS medium supplemented with different plant hormones. Chromosome Analysis of Regenerated Plant by Conventional Compression and Microscopy. Results: Callus induction was the best with MS + 2,4-D 1.0 mg.L-1 + KT 2.0 mg.L-1, and callus induction rate was 41.07%. MS + 6-BA 1.5-2.0 mg.L-1 + NAA 0.1-0.3 mg.L-1 was suitable for the induction of adventitious buds, and adventitious buds were transferred to 1/2 MS of NAA 0.1-0.3 mg.L-1 Rooting medium, after rooting to obtain a complete regeneration of plants, regenerated plants for somatic origin. At the same time, the effect of low temperature pretreatment at 4 ℃ on callus induction was discussed. Conclusion: The establishment of Hubei Ophiopogon japonicus somatic cell culture system and rapid propagation.