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目的 :克隆出正确的人源性角质细胞生长因子 (KGF)并探讨影响其表达的因素。方法 :应用RT_PCR技术克隆出KGF基因 ,并用pBV2 2 0表达质粒对其进行表达 ,然后用RNAdraw对其RNA进行分析。结果 :带有信号肽的KGF基因在pBV2 2 0中表达量为 10 %左右 ,而去掉信号肽的KGF基因在pBV2 2 0中表达量估计只有 1%～ 2 % ;利用RNAdraw分析的结果表明前者RNA前端没有形成茎环结构 ,而后者则形成了茎环结构。结论 :KGF基因在细菌中表达量较低 ,信号肽是影响其表达量的重要因素
OBJECTIVE: To clone the correct human keratinocyte growth factor (KGF) and to explore the factors influencing its expression. Methods: KGF gene was cloned by RT_PCR technique and expressed by pBV220 expression plasmid. The RNA was analyzed by RNAdraw. Results: The expression of KGF gene with signal peptide was about 10% in pBV220 and the expression level of KGF gene with signal peptide removed in pBV220 was only 1% -2%. The results of RNAdraw analysis showed that the former The stem-loop structure was not formed at the RNA front end, whereas the latter formed a stem-loop structure. Conclusion: The expression of KGF gene in bacteria is low, and signal peptide is an important factor affecting its expression