目的采用高效液相色谱法测定顺铂注射液中顺铂的含量。方法以Hypersil NH2 色谱柱 ,0 .1%NaCl溶液为流动相 ,流速为 0 .8ml/min ,顺铂和反铂的保留时间分别为 3.37和 5 .5 1min ,检测波长为 2 10nm、最低检测限为 6ng。结果标准曲线的线性范围为 10 0 .0 4～ 30 0 .12 μg/ml(r=0 .9997) ,方法回收率为 99.0 7% ,RSD为 0 .5 4 %。结论此法可用于直接测定顺铂注射液中顺铂的含量 Objective To determine the content of cisplatin in cisplatin injection by high performance liquid chromatography. Methods The mobile phase consisted of Hypersil NH2 column and 0.1% NaCl solution with a flow rate of 0.8ml / min. The retention times of cisplatin and transplatin were 3.37 and 5.51min respectively. The detection wavelength was 210nm. The lowest detection Limit 6ng. Results The calibration curve was linear over the range of 10 0 .04 to 30 0 .12 μg / ml (r = 0.9997). The recovery was 99.0 7% and the RSD was 0.54%. Conclusion This method can be used for direct determination of cisplatin injection cisplatin content