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目的:研究宫颈癌中MMP-2、MMP-9、Ⅳ型胶原表达和微血管密度的临床意义。方法:采用SABC免疫组织化学方法对64例子宫颈癌组织(全部为鳞癌),20例正常宫颈组织进行MMP-2、MMP-9,Ⅳ型胶原和微血管密度的检测。结果:(1)MMP-2,MMP-9二者在正常宫颈组织、宫颈癌(ICC)的细胞浆的阳性表达率分别为:MMP-2阳性20.0%(4/20)、75.0%(48/64);MMP-9的阳性为:15.0%(3/20)、78.1%(50/64),MMP-2、MMP-9的正常组与宫颈癌组相比差异有显著性(p<0.05)。(2)Ⅳ型胶原表达随着宫颈癌的进展、分期升级,基底膜破坏程度明显增加而显著减少,正常组织与宫颈癌之间差异显著。有淋巴结转移者Ⅳ型胶原表达则明显低于无淋巴结转移组(p<0.005)。(3)微血管密度与宫颈癌的恶性程度及淋巴结转移密切相关,宫颈癌患者的微血管密度明显高于对照组,在临床II、III期明显高于I期。MVD与肿瘤大小、病理分级无关。结论:MMP-2、MMP-9活性表达与Ⅳ型胶原的表达负相关,与MVD正相关。Ⅳ型胶原的表达与宫颈癌的临床分期及有无淋巴结转移相关,可作为判定宫颈癌浸润及转移程度的一个生物学指标。微血管密度与宫颈癌的恶性程度及淋巴结有无转移密切相关。因此采用MVD评价宫颈癌预后是有意义的。
Objective: To study the clinical significance of the expression of MMP-2, MMP-9, type Ⅳ collagen and microvessel density in cervical cancer. Methods: SABC immunohistochemistry was used to detect MMP-2, MMP-9, type IV collagen and microvessel density in 64 cases of cervical cancer (all squamous cell carcinoma) and 20 cases of normal cervical tissue. Results: (1) The positive expression rates of MMP-2 and MMP-9 in cytoplasm of normal cervical tissue and cervical cancer were 20.0% (4/20) and 75.0% (48/20) respectively / 64). The positive rate of MMP-9 was 15.0% (3/20) and 78.1% (50/64) respectively. The difference between the normal group and the cervical cancer group was statistically significant (p < 0.05). (2) The expression of type Ⅳ collagen with the progress of cervical cancer, stage upgrade, basement membrane destruction increased significantly significantly reduced, significantly different between normal tissues and cervical cancer. The expression of type Ⅳ collagen in patients with lymph node metastasis was significantly lower than those without lymph node metastasis (p <0.005). (3) The microvessel density is closely related to the malignant degree of cervical cancer and lymph node metastasis. The microvessel density of cervical cancer patients is obviously higher than that of control group, which is obviously higher than that of stage I and II in clinical stage. MVD and tumor size, pathological grade has nothing to do. Conclusion: The expressions of MMP-2 and MMP-9 are negatively correlated with the expression of type Ⅳ collagen and positively correlated with MVD. The expression of type Ⅳ collagen is correlated with the clinical stage and lymph node metastasis of cervical cancer, which can be used as a biological index to determine the degree of cervical cancer infiltration and metastasis. Microvessel density and the degree of cervical cancer and lymph node metastasis are closely related. Therefore, the use of MVD evaluation of cervical cancer prognosis is meaningful.