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目的 通过研究NF κB与感染性早产的关系 ,探索感染性早产的发病机制。方法 将 45只孕 15d的小鼠分为LPS组、LPS +PDTC(pyrrolidinedithiocarbamate)组、生理盐水组 3组 ,每组 15只 ,分别注药后观察它们的分娩时间 ,另外同样分组孕鼠 ,每组 5只 ,共 15只 ,在给予上述同样药物 16h后处死 ,以免疫组化技术检测其胎盘TNF α、IL 8水平。结果 LPS +PDTC组孕鼠的妊娠时间较LPS组明显延长 ,另 3组孕鼠检测TNF α、IL 8水平 ,发现在LPS +PDTC组中 ,其表达较LPS组明显降低。结论 NF κB活化可能是通过调控某些细胞因子的表达 ,参与感染性早产的发生。
Objective To explore the pathogenesis of infectious preterm labor by studying the relationship between NF κB and infectious premature birth. Methods 45 pregnant 15-day-old mice were divided into LPS group, LPS + PDTC (pyrrolidineithiocarbamate) group and normal saline group (15 rats in each group), and their delivery time was observed after injection. A total of 15 rats in group A were sacrificed 16h after the same drugs were given. The levels of TNFα and IL-8 in placenta were detected by immunohistochemistry. Results Pregnant mice in LPS + PDTC group had significantly longer pregnancy time than those in LPS group. The levels of TNFα and IL-8 in the other three groups of pregnant rats were significantly lower than those in LPS + PDTC group. Conclusion The activation of NF-κB may be involved in the occurrence of infectious premature labor by regulating the expression of certain cytokines.