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为探究半野生棉群体材料的遗传变异和群体结构,挖掘与含油量相关的标记位点,利用215对SSR引物,对89份半野生棉自然群体进行遗传多样性、群体结构和亲缘关系分析,并进行棉仁含油量性状与标记的关联分析。结果表明,位点多态信息含量(PIC)为0.0222~0.7390,平均为0.3452;基因型多样性(H′)介于0.0616~1.4412,平均为0.5614。群体结构分析将89份材料分为2个亚群,群体结构简单,遗传变异比较丰富,可以用于半野生棉目标性状的关联分析。应用关联分析,在3个年份环境下重复检测出12个与棉仁含油量显著相关的位点(P<0.05),这12个位点可能与含油量性状存在稳定的关联,可为棉仁含油量性状的分子标记辅助选择提供依据,为开展半野生棉其它性状的关联分析提供参考。阔叶棉141平均棉仁含油量为41.90%,为高油份含量品种的遗传改良提供理想材料。
In order to explore the genetic variation and population structure of semi-wild cotton population, the marker sites related to oil content were excavated. 215 pairs of SSR primers were used to analyze the genetic diversity, population structure and genetic relationship among 89 wild semi- The correlation analysis between the traits and markers of oil content of cottonseed was carried out. The results showed that the polymorphic information content (PIC) of loci was 0.0222-0.7390 with an average of 0.3452. The genotype diversity (H ’) ranged from 0.0616 to 1.4412 with an average of 0.5614. Population structure analysis 89 materials were divided into two subgroups, the population structure is simple, genetic variation is rich, can be used for correlation analysis of semi-wild cotton target traits. Using correlation analysis, 12 sites (P <0.05) significantly correlated with oil content of cottonseed were detected repeatedly in three years. These 12 sites may be related to the oil content stablely, Provide a basis for molecular marker-assisted selection of oil traits, and provide references for carrying out the correlation analysis of other traits of semi-wild cotton. The average cottonseed oil content of broadleaf 141 was 41.90%, which provided the ideal material for the genetic improvement of high oil content varieties.