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目的探讨浙江省急性腹泻患儿的常见病毒组成。方法采集5岁以下(≤5岁)急性腹泻患儿的811份粪便标本,采用ELISA方法检测A组轮状病毒(RV),应用多重PCR方法同时检测B/C组RV、诺如病毒(NoV)、札如病毒(SaV)、肠道腺病毒(AdV)、星状病毒(AstV),并利用RT-PCR对RV的阳性标本进行G、P分型。结果811份粪便样本中,RV、NoV、SaV、AdV及AstV总的阳性比例分别为25.52%、18.37%、4.44%、2.71%和1.23%;65份(8.01%)混合感染标本中以RV和NoV混合感染所占比例最大(56.92%);116份RV的G/P分型中,G3型(37.93%)、G1型(32.75%)和P[8]型(57.76%)是RV的优势型别,且G3/P[8](27.59%)是混合感染最主要的组合。结论浙江省五种常见腹泻病毒及其混合感染的流行状况,为临床治疗和疾病监测提供参考价值。
Objective To investigate the common virus components in children with acute diarrhea in Zhejiang Province. Methods 811 stool specimens from children with acute diarrhea under 5 years old (≤5 years old) were collected. The rotavirus (A) of group A was detected by ELISA. RV, norovirus (NoV (SaV), adenovirus (Enterovirus), and Astrovirus (AstV). RT-PCR was used to detect G and P genotypes in positive specimens of RV. Results The total positive rates of RV, NoV, SaV, AdV and AstV in 811 stool samples were 25.52%, 18.37%, 4.44%, 2.71% and 1.23%, respectively. Among 65 samples (8.01% NoV mixed infection accounted for the largest proportion (56.92%); 116 RV G / P classification, G3 (37.93%), G1 (32.75%) and P [8] (57.76% Type, and G3 / P [8] (27.59%) is the most important combination of mixed infection. Conclusion The prevalence of five common diarrhea viruses and their mixed infections in Zhejiang Province provide reference value for clinical treatment and disease surveillance.