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目的诱导复发性实验性自身免疫性葡萄膜炎(experimental autoimmune uveoretinitis,EAU)小鼠模型,评价其发生过程及特点。方法完全弗氏佐剂(CFA)乳化的光感受器间维生素A类结合蛋白(IRBP)161-180肽段免疫B10.RⅢ小鼠作为诱导组(35只),用CFA-PBS免疫小鼠作为对照组(6只)。小鼠免疫后7、14、21、28、35 d裂隙灯显微镜和光学显微镜观察及评价眼部炎症发生、眼部表现和病理学变化;待炎症完全消失时,再次免疫小鼠,评价小鼠眼部炎症复发。结果诱导组首次免疫后7 d出现初发炎症,14 d炎症达高峰,随后炎症消退,至35 d完全消失。第35天进行再次免疫,36 d出现复发炎症,42 d达炎症高峰,随后炎症消退,但至观察期第96天部分鼠(1/5)仍维持炎症。眼部表现为睫状充血、前房渗出、瞳孔受损。病理学表现为视网膜和脉络膜炎性细胞浸润,血管炎、肉芽肿性炎,光感受器细胞层破坏,视网膜下渗出。对照组未见明显炎症。结论建立的EAU呈现慢性复发性病程,其眼部表现和病理学特征与人类葡萄膜炎相似,可作为葡萄膜炎研究的新型动物模型。
Objective To induce a mouse model of recurrent experimental autoimmune uveoretinitis (EAU) and evaluate its occurrence and characteristics. Methods B10.RⅢ mice were immunized with CFA emulsified photoreceptor vitamin A binding protein (IRBP) 161-180 peptide as an induction group (35 mice) and mice were immunized with CFA-PBS as a control Group (6). Mice were immunized at 7, 14, 21, 28, 35 days after Slit lamp and optical microscopy to observe and evaluate the occurrence of ocular inflammation, ocular manifestations and pathological changes. When the inflammation completely disappeared, the mice were immunized again to evaluate the mice Eye inflammation recurrence. Results In the induction group, initial inflammation occurred on the 7th day after the first immunization, and peaked on the 14th day. The inflammation subsided and disappeared completely after 35 days. On the 35th day of re-immunization, relapse inflammation occurred on the 36th day and peaked on the 42nd day. Inflammation then subsided, but some rats (1/5) maintained the inflammation on the 96th day of the observation period. Ciliary congestion of the eye performance, anterior chamber exudation, impaired pupil. Pathological manifestations of retinal and choroidal inflammatory cell infiltration, vasculitis, granulomatous inflammation, photoreceptor cell layer destruction, subretinal exudation. No obvious inflammation in the control group. Conclusion The established EAU has a chronic recurrent course with similar ocular and pathological features as human uveitis and may serve as a novel animal model for uveitis.