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本文报道了中华猕猴桃(Actinidia chinensis Planch.var.chinensis C.F.Liang)及硬毛猕猴桃 (A.chinensis Planch.var.hispida C.F.Liang)雌、雄株离体茎段的脱分化和植株再生。在以MS为基本培养基,补加BAP(0.5,2ppm),IAA(0.05ppm)或玉米素(0.1—10ppm)的培养基上,得到了愈伤组织及再分化出苗,其中,以MS补加1ppm玉米素的效果最为显著。中华猕猴桃和硬毛猕猴桃产生愈伤组织的能力近似,但在诱导苗的频率上,硬毛猕猴桃远较中华猕猴桃为高;在雌、雄株之间,雌株又较雄株的高。当小苗的茎伸长至1厘米以上时,可切下置于50ppm IBA溶液中浸泡2小时或2ppm IBA溶液中浸24小时,而后再转入1/2 MS,1％蔗糖及0.5％活性炭的生根培养基上诱导生根。从我们的实验结果看,利用离体茎段的组织培养以诱导植株再生的方法,有可能作为繁殖猕猴桃雌株无性系及扩大优良单株的一种手段。
In this paper, the dedifferentiation and plant regeneration of in vitro stem segments of Actinidia chinensis Planch.var.chinensis C.F.Liang and A.chinensis Planch.var.hispida C.F.Liang were reported. Callus and redifferentiated shoots were obtained on MS medium supplemented with BAP (0.5, 2 ppm), IAA (0.05 ppm) or zeatin (0.1-10 ppm) The effect of adding 1ppm zeatin is the most significant. Actinidia chinensis and Actinidia chinensis had the similar ability to produce callus, but the frequency of inducing seedlings was much higher than that of Actinidia chinensis. The female and male plants were higher than the male plants. When the stems of the seedling are elongated to more than 1 cm, they can be cut into 2-hour immersion in a 50ppm IBA solution or 2-minute immersion in a 2-ppm IBA solution for 24 hours, then transferred to 1/2 MS, 1% sucrose and 0.5% activated carbon Rooting medium induces rooting. From our experimental results, it is possible to use the method of tissue culture of in vitro stem segments to induce plant regeneration, which may be used as a means of propagating clonal kiwifruit female clones and expanding superior individuals.