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为探讨不同粒径纳米硒化镉对雄性小鼠精子质量的影响,将30只SPF级雄性昆明小鼠随机分为3组,每组10只,即对照(生理盐水)组、大粒径(50~100 nm)纳米CdSe组、小粒径(40~50 nm)纳米CdSe组,以1/20半数致死量(LD50,即40 mg/kg)对小鼠进行染毒。采用腹腔注射方式进行染毒,染毒容量为10 ml/kg,隔日1次,连续30 d。染毒结束后,统计精子成活率;采用放射免疫分析法检测血清睾酮含量。结果显示,与对照组相比,各粒径纳米CdSe组小鼠血清中睾酮含量和精子存活率均降低,差异有统计学意义(P<0.05)。与小粒径纳米CdSe组比较,大粒径纳米CdSe组小鼠精子存活率较高,差异有统计学意义(P<0.05)。提示纳米硒化镉可严重影响精子的生成,其中,小粒径纳米硒化镉的作用较强。
In order to investigate the effect of nanometer cadmium selenide with different particle size on the sperm quality of male mice, 30 SPF male Kunming mice were randomly divided into 3 groups with 10 rats in each group, namely control (saline) group, 50 ~ 100 nm) CdSe group and small particle size (40 ~ 50 nm) CdSe group. The mice were exposed to 1/20 LD50 (40 mg / kg). The rats were injected intraperitoneally with the dose of 10 ml / kg every other day for 30 days. After the exposure, the survival rate of sperm was calculated. The serum testosterone level was detected by radioimmunoassay. The results showed that compared with the control group, the serum testosterone content and sperm survival rate of the CdSe nanoparticles of various particle sizes decreased, the difference was statistically significant (P <0.05). Compared with small particle size CdSe group, the survival rate of sperm in large particle size CdSe group mice was higher, the difference was statistically significant (P <0.05). It is suggested that nano-CdSe can seriously affect sperm production. Among them, the effect of nano-CdSe with small particle size is stronger.