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目的:探讨组胺处理体外培养的皮肤鳞状细胞癌细胞(A431)对其生物学行为的影响。方法:用细胞活力(CCK-8)法检测不同浓度组胺处理A431细胞的活性,筛选用于本研究的有效组胺浓度。流式细胞仪检测癌细胞增殖周期细胞百分比与细胞增殖指数;划痕、Transwell小室和黏附实验分别检测癌细胞的迁移、侵袭和黏附潜能。结果:与未处理的对照组相比,组胺处理A431细胞在48 h出现显著抑制作用,呈浓度和时间依赖关系。组胺10~4 mol/L组在48 h和72 h处理癌细胞活力比其他浓度组更显著(均P<0.01)。组胺10~4mol/L组处理后细胞在G1期比率显著增加,在S期比率明显降低(均P<0.05),并抑制A431细胞的迁移、侵袭和黏附(均P<0.05)。结论:组胺能有效抑制A431细胞增殖、迁移、侵袭和黏附的生物学行为。
Objective: To investigate the effect of histamine treatment on the biological behavior of human skin squamous cell carcinoma (A431) cultured in vitro. Methods: The activity of histamine-treated A431 cells was tested by cell viability assay (CCK-8), and the effective histamine concentration was screened for this study. Flow cytometry was used to detect the percentages of cell cycle and proliferation index of cancer cells. Scratches, Transwell chambers and adhesion assays were used to detect the migration, invasion and adhesion potential of cancer cells respectively. Results: Compared with the untreated control group, histamine A431 cells significantly inhibited at 48 h in a time-and concentration-dependent manner. The viability of cancer cells treated with histamine at 10 ~ 4 mol / L for 48 h and 72 h was significantly higher than that of other concentrations (all P <0.01). After treated with 10 ~ 4mol / L histamine, the percentage of cells in G1 phase increased significantly, while the ratio in S phase decreased significantly (all P <0.05) and inhibited the migration, invasion and adhesion of A431 cells (all P <0.05). Conclusion: Histamine can effectively inhibit the biological behavior of A431 cells in proliferation, migration, invasion and adhesion.