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作者用HPLC测定了小鼠组织和体液中的强力霉素及其代谢产物:N-去甲强力霉素(下简称N-D)。将雌性小鼠分为对照组和诱导组,后者腹腔注射苯巴比妥35mg/kg,以诱导药物代谢酶,每天二次,给药三天;对照组小鼠给予等量生理盐水。预处理后,二组小鼠均静脉注射强力霉素50mg/kg。在给药后不同间隔内(2～20h)处死小鼠,取组织和体液样品。另用二组小鼠的肝脏微粒体研究体外强力霉素去甲基化作用,并借乙基吗啡去甲基化作用来鉴定此体外试验的有效性。
The authors used the determination of doxorubicin and its metabolite in mice tissues and body fluids: N-desmethoxycin (hereinafter referred to as N-D). Female mice were divided into the control group and the induction group, which were injected intraperitoneally with phenobarbital 35mg / kg to induce drug-metabolizing enzymes twice daily for three days. The control group mice were given the same amount of normal saline. After pretreatment, two groups of mice were intravenously injected with doxycycline 50mg / kg. Mice were sacrificed at different intervals (2 ~ 20h) after administration, and tissue and body fluid samples were taken. In addition, two groups of mice were used to study the demethylation of doxycycline in vitro and the demethylation of demethylation of emodin to identify the effectiveness of this in vitro test.