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[Objective] The study aimed to explore the effects of Zizhuliqing Oral Liquid on animal cellular immune function. [Method] MTT method and phagocytizing natural red method were used to determine the effects of Zizhuliqing Oral Liquid on piglet lymphocyte transformation and the phagocytosis of mouse peritoneal macrophages respectively. [Result] The lymphocyte transformation rates of piglets in medicated groups were significantly higher than that in control group; the difference of mouse peritoneal macrophage activities between the medicated groups and the control group was obvious. [Conclusion] Zizhuliqing Oral Liquid could promote the transformation of piglet T lymphocytes induced by ConA and the phagocytosis of mouse peritoneal macrophages to natural red, indicating its good immune enhancement function.