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目的 :研究平滑肌细胞增殖和凋亡在新生乳猪生后正常发育及缺氧性肺动脉高压肺血管重构中的作用。方法 :4 2只新生乳猪分成正常发育组和缺氧性肺动脉高压组 ,其中 2 0只新生乳猪置于低气压仓 (5 0 .8kPa)中 3或11d形成不同程度的缺氧性肺动脉高压模型。采用免疫组化方法和TUNEL方法检测和观察肺小阻力动脉中层平滑肌细胞 (SMC)的增殖和凋亡变化。结果 :平滑肌细胞复制率在出生时即较高 ,出生后早期进一步升高。短期缺氧对平滑肌细胞复制率有一定程度的抑制。较长期缺氧 (11d)引起肺血管重构显著变化同时 ,平滑肌细胞复制率显著增加 ;新生期无论在正常状态 ,缺氧或缺氧恢复阶段 ,在肺血管壁上均未见细胞凋亡阳性信号。结论 :新生乳猪出生后肺血管重构与平滑肌细胞增殖有关。平滑肌细胞增殖在严重缺氧性肺动脉高压形成中起重要作用。细胞凋亡在出生早期肺血管重构中作用尚待研究
OBJECTIVE: To study the role of proliferation and apoptosis of smooth muscle cells in normal neonatal piglets postnatal development and pulmonary vascular remodeling in hypoxic pulmonary hypertension. Methods: 42 newly born piglets were divided into normal developmental group and hypoxic pulmonary hypertension group. Among them, 20 newborn piglets were placed in low pressure chamber (5.08kPa) for 3 or 11 days to form hypoxic pulmonary artery High-pressure model. Immunohistochemical method and TUNEL method were used to detect and observe the changes of proliferation and apoptosis of SMC in small pulmonary artery. RESULTS: Smooth muscle cell replication was higher at birth and further increased at early postnatal day. Short-term hypoxia inhibited the smooth muscle cell replication to a certain degree. Longer-term hypoxia (11d) caused significant changes in pulmonary vascular remodeling at the same time, significantly increased the rate of smooth muscle cell replication; Neonatal period in normal conditions, hypoxia or hypoxia recovery stage, no pulmonary vascular wall in the positive apoptotic signal. Conclusion: Pulmonary vascular remodeling of neonatal suckling piglets is related to the proliferation of smooth muscle cells. Smooth muscle cell proliferation plays an important role in the formation of severe hypoxic pulmonary hypertension. The role of apoptosis in early pulmonary angiogenesis remains to be studied