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为了探明吉林省不同时期育成的栽培大豆品种在叶片衰老过程中保护酶活性、丙二醛和可溶糖含量的变化,将1923-2006年划分为四个时期,第一时期(1960年以前)、第二时期(1961-1980年)、第三时期(1981-2000年)和第四时期(2001年以后),以吉林省不同时期育成的20个栽培大豆品种为材料进行田间试验.分别在R2(开花期)、R4(结荚期)、R6(鼓粒期)、R7(成熟期)取样测定超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性,以及丙二醛(MDA)和可溶性糖含量.结果表明,吉林省不同时期育成的大豆品种叶片衰老过程中SOD,CAT活性随着生育时期的递进,呈现先升高后下降的趋势,在鼓粒期最大,成熟期最小.而POD活性、可溶性糖和丙二醛含量随着生育时期的推进呈上升趋势变化,在成熟期达到最大值.不同时期育成的大豆品种保护酶活性均表现为第一时期＜第二时期＜第三时期＜第四时期.可溶性糖含量在成熟期表现为:第一时期＜第二时期＜第四时期＜第三时期,而丙二醛含量在成熟期表现为第四时期＜第三时期＜第二时期＜第一时期.这为调控延缓叶片的衰老,进行高产品种选育及栽培提供理论参考.“,”The aim of this study was to investigate the changes of protective enzyme activities,malondialdehyde contents and soluble sugar contents during leaves senescence of soybean cultivars grown in different periods in Jilin province.In this research,1923-2006 were divided into four periods,the first period was before 1960,the second period was 1961 to 1980,the third period was 1981-2000 and the fourth period was after 2001.20 soybean varieties fostered in different periods in Jilin province were taken as experimental materials to measure field trials.The activities of superoxide dismutase (SOD),peroxidase (POD),catalase (CAT),the contents of malondialdehyde (MDA) and soluble sugar were measured by sampling from R2 (flowering stage),R4 (podding stage),R6 (granule stage) and R7 (maturation stage),respectively.The results showed that the activities of SOD and CAT increased firstly and then decreased with promotion of growth period in the process of leaves senescence of soybean cultivars in different periods in Jilin province,which reached the maximum at granule stage and the minimum at mature stage.The POD activities and the contents of soluble sugar and malondialdehyde (MDA) gradually increased with the promotion of growth period,and reached the maximum at maturity.The activities of protective enzymes in soybean cultivars at different times showed the first breeding period ＜the second breeding period ＜the third breeding period＜the fourth breeding period,soluble sugar contents at maturity showed the first breeding period＜ the second breeding period ＜the fourth breeding period ＜the third breeding period,and the malondialdehyde contents at maturity showed the fourth breeding period＜the third breeding period＜the second breeding period＜the first breeding period.This provided a theoretical basis for delaying the leaves senescence and improving the breeding and cultivation of high-yielding varieties.