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为探究不同桔小实蝇Bactrocera dorsalis(Hendel)地理种群的遗传变异、入侵来源和扩散情况,利用13对引物对中国南方10省区、泰国、夏威夷、菲律宾和老挝的30个桔小实蝇种群共180个个体的遗传多样性水平进行了研究。Popgene32和NTSYS-pc2.10e软件分析结果表明:30个不同桔小实蝇种群的遗传相似度在0.3599~0.9153范围内。种群的Nei氏基因多样性指数平均为0.6464±0.1026,Shannon信息指数I平均为1.2845±0.2632,提示桔小实蝇种群具有较丰富的遗传多样性。UPGMA聚类分析显示,福建地区和海南地区分别独立一支,广东地区和台湾地区种群聚成一支,而广西、泰国、湖南、云南、老挝、四川、重庆和贵州地区聚为一大支系。据此提出泰国种群和老挝种群是最早入侵我国的种群,云南地区是最早的入侵地,广西地区可能为又一较早入侵地。
In order to explore the genetic variation, invasion origin and spread of the geographic populations of Bactrocera dorsalis (Hendel) from different regions of China, Thirteen pairs of primers were used to detect the genetic diversity of 30 populations of ten Tricholoma matsutake populations in ten southern provinces of China, Thailand, Hawaii, Philippines and Laos A total of 180 individuals were studied for their genetic diversity. The results of Popgene32 and NTSYS-pc2.10e softwares showed that the genetic similarity of 30 different fruit fly populations was in the range of 0.3599 ~ 0.9153. The average Nei’s gene diversity index of population was 0.6464 ± 0.1026, Shannon’s information index I was 1.2845 ± 0.2632, which indicated that the population of orange fruit fly had rich genetic diversity. UPGMA cluster analysis showed that Fujian and Hainan were independent, while Guangdong and Taiwan had one population, while Guangxi, Thailand, Hunan, Yunnan, Laos, Sichuan, Chongqing and Guizhou clustered together. Accordingly, it is suggested that the Thai population and the Lao population are the earliest invaders in our country. Yunnan is the earliest invaded land and the Guangxi area may be another earlier invasion.