In this study,a coniferous tree species(Pinus tabuliformis Carr.) was investigated at a moderate-altitude mountainous terrain on the southern slope of the middle Qinling Mountains(QLM) to detect the trends in carbon isotope ratio( δ~(13)C),leaf nitrogen content(LNC) and stomatal density(SD) with altitude variation in northsubtropical humid mountain climate zone of China.The results showed that LNC and SD both significantly increased linearly along the altitudinal gradient ranging from 1000 to 2200 m,whereas leafδ~(13)C exhibited a significantly negative correlation with the altitude.Such a correlation pattern differs obviously from that obtained in offshore low-altitude humid environment or inland high-altitude semi-arid environment,suggesting that the pattern of increasing δ~(13)C with the altitude cannot be generalized.The negative correlation between δ ~(13)C and altitude might be attributed mainly to the strengthening of carbon isotope fractionation in plants caused by increasing precipitation with altitude.Furthermore,there was a remarkable negative correlation between leaf δ ~(13)C and LNC.One possible reason was that increasing precipitation that operates to increase isotopic discrimination with altitude overtook the LNC in determining the sign of leaf δ ~(13)C.The significant negative correlation between leaf δ ~(13)C and SD over altitudes was also found in the present study,indicating that increases in SD with altitude would reduce,rather than enhance plant δ~(13)C values. In this study, a coniferous tree species (Pinus tabuliformis Carr.) Was investigated at a moderate-altitude mountainous terrain on the southern slope of the middle Qinling Mountains (QLM) to detect the trends in carbon isotope ratio (δ ~ (13) C ), leaf nitrogen content (LNC) and stomatal density (SD) with altitude variation in northsubtropical humid mountain climate zone of China. The results showed that LNC and SD both significant increased linearly along the altitudinal gradient ranging from 1000 to 2200 m, ~ (13) C exhibited a significant negative correlation with the altitude. Shuch a correlation pattern differs obviously from that obtained in offshore low-altitude humid environment or inland high-altitude semi-arid environment, suggesting that the pattern of increasing δ ~ (13) ) C with the altitude can not be generalized. The negative correlation between δ ~ (13) C and altitude might be attributed mainly to the strengthening of carbon isotope fractionation in plants caused by increasi ng precipitation with altitude. Futuremore, there was a remarkable negative correlation between leaf δ ~ (13) C and LNC. One possible reason was was increasing precipitation that operates to increase isotopic discrimination with altitude overtook the LNC in determining the sign of leaf δ ~ (13) C. The significant negative correlation between leaf δ ~ (13) C and SD over altitudes was also found in the present study, indicating that increases in SD with altitude would reduce, rather than enhance plant δ ~ (13) C values .