论文部分内容阅读
目的:建立以反相高效液相色谱法测定神曲胃痛胶囊中大黄素、大黄酚含量的方法。方法:色谱柱为Phenomenex BDS-C18(250mm×4.6mm,5μm),流动相为乙腈-水-冰醋酸(55∶45∶0.5),流速为1.0mL.min-1,检测波长为254nm。结果:大黄素、大黄酚的检测浓度分别在2.188~54.700、4.312~107.800μg.mL-1范围内与各自峰面积积分值呈良好的线性关系(r均为0.9999);二者平均回收率分别为96.52%、98.45%,RSD分别为0.59%、0.43%。结论:本方法简便、可靠、重现性好,可用于神曲胃痛胶囊的质量控制。
Objective: To establish a method for the determination of emodin and chrysophanol in Shenqi Weitong Capsules by RP-HPLC. Methods: The chromatographic column was Phenomenex BDS-C18(250mm×4.6mm, 5μm). The mobile phase was acetonitrile-water-glacial acetic acid (55∶45∶0.5). The flow rate was 1.0mL.min-1. The detection wavelength was 254nm. Results: The detection concentrations of emodin and chrysophanol in the range of 2.188~54.700 and 4.312~107.800μg.mL-1 showed a good linear relationship with their respective peak area integrals (r=0.9999); the average recoveries of the two were respectively It was 96.52% and 98.45%, and the RSD was 0.59% and 0.43% respectively. Conclusion: This method is simple, reliable, and reproducible. It can be used for the quality control of Shenqi Weitong Capsule.