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以十二指肠钩虫和美洲钩虫感染期幼虫及成虫粗抗原,应用ELISA检测感染小鼠及钩虫感染者的血清抗体发现小鼠感染十二指肠钩虫后d_7已出现抗体;d_(15～30)抗体水平达高峰;小鼠感染美洲钩虫后d_5,出现抗体,d_(10)抗体水平达高峰,此后均逐渐降低。ELISA检测钩虫感染者血清抗体具有较高的敏感性和特异性。
The larvae of A. hooker and the crude antigen of adult hookworm infected with hookworm and hookworm were detected by ELISA. Serum antibodies of infected and hookworm infected mice were detected by ELISA. Antibody was found on mice infected with hookworm duodenum. D_ (15 ~ 30 ) Antibody reached its peak. After the mice were infected with hookworm, the antibody appeared on day d_5, and the level of d_ (10) antibody reached its peak, then decreased gradually. ELISA detection hookworm infected serum antibodies with high sensitivity and specificity.