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目的比较倾注法与涂布法接种培养菌数的差异。方法采用倾注接种法和涂布接种法进行活菌计数培养,观察平板上菌落形态和菌落数的差异。结果倾注接种法培养的平板上部分在培养基深层的菌落形态变异或显小;而涂布接种法培养的平板上菌落形态一致,易辨认。倾注接种法和涂布接种法培养的平板上菌落数之间无差异。结论倾注法和涂布法接种培养细菌数无差异,但涂布法对人员接种技术要求高。
OBJECTIVE To compare the differences in the number of inoculated cultures between the two methods. Methods Pour inoculation method and coating inoculation method were used to count the number of viable cells. The difference of the colony morphology and the number of colonies on the plate was observed. Results Inoculation method in the upper part of the culture plate in the colonial morphology of sub-variant or small; and coated inoculation culture plate flat colonies consistent and easy to identify. There was no difference between the number of colonies on the plate by inoculation and coating inoculation. Conclusion There is no difference in the number of inoculated bacteria cultured by pouring method and coating method, but the coating method has high requirements on the inoculation technique.