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目的:研究玉郎伞多糖(YLSP)对Aβ25-35诱导PC12细胞凋亡的保护作用。方法:采用Aβ25-35诱导PC12细胞损伤凋亡模型,给予YLSP预处理后,MTT比色法分析细胞存活率,倒置相差显微镜、透射电镜观察细胞形态改变,以观察药物的保护作用。结果:MTT法显示与正常对照组相比较,模型组细胞存活率显著下降(P<0.01),与模型组比较,YLSP各浓度组均使细胞存活率显著升高(P<0.05或P<0.01);倒置相差显微镜、透射电镜观察显示Aβ25-35可导致PC12细胞凋亡,经YLSP处理后,模型细胞凋亡情况明显改善。结论:YLSP对Aβ25-35诱导的PC12细胞凋亡有保护作用。
Objective: To study the protective effect of YLSP on Aβ25-35 induced PC12 cell apoptosis. Methods: The apoptotic model of PC12 cells was induced by Aβ25-35. After being pretreated with YLSP, cell viability was analyzed by MTT colorimetric assay. The morphological changes of cells were observed by inverted phase contrast microscope and transmission electron microscope to observe the protective effect of the drugs. Results: Compared with the normal control group, the cell viability of the model group was significantly decreased (P <0.01) by MTT assay. Compared with the model group, the cell survival rate was significantly increased in all concentrations of YLSP group (P <0.05 or P <0.01) ); Inverted phase contrast microscope, transmission electron microscopy showed Aβ25-35 can lead to PC12 cell apoptosis, the YLSP treatment, the model of apoptosis was significantly improved. Conclusion: YLSP can protect PC12 cells from Aβ25-35-induced apoptosis.