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目的:探讨心脏中诱导性一氧化氮合酶(induciblenitricoxidesynthase,iNOS)与心肌缺血性损伤和β-肾上腺受体兴奋之间的关系。方法:实验选用雄性Fisher大鼠50只。随机将其中20只大鼠(鼠龄3～5个月)分为青年大鼠对照组和青年大鼠干预组各10只;20只老年大鼠(鼠龄24～25个月)分为老年大鼠对照组和老年大鼠干预组各10只。余下年轻和老年大鼠各5只,处死后,取心肌组织作一氧化氮合酶Westernblot研究。青年大鼠对照组大鼠和老年大鼠对照组大鼠实验前接受生理盐水干预,青年大鼠干预组和老年大鼠干预组大鼠接受特异性iNOS阻断剂-1400W干预。4组大鼠的离体心脏分别接受生理盐水和1400W干预及50%正常冠状动脉流量灌注30min后,泵入异丙肾上腺素(isoproterenol,Iso)30min。结果:心肌梗死后30min,所有组左心室形成压leftventriculardeveloped(pressure,LVDP),左室内压最大上升下降速率rateofriseofleftventric-(ularpressure,±dp/dt)均较基础值下降13%～45%。Iso泵入青年大鼠对照组和青年大鼠干预组大鼠30min后,心室功能部分恢复,表现为LVDP和±dp/dt上升。与此明显对比,Iso泵入老年大鼠对照组大鼠后,非但未改善缺血所致的心功能不全,反倒进一步加重心功能损伤。表现为LVDP和±dp/dt进一步下降43%～60%。Westernblot研
Objective: To investigate the relationship between inducible nitric oxide synthase (iNOS) and myocardial ischemic injury and β-adrenergic receptor excitability in the heart. Methods: Fifty male Fisher rats were selected. Twenty rats (3-5 months old) were randomly divided into 10 groups: the young rats control group and the young rats intervention group; 20 old rats (24-25 months old) were divided into old age The rats in the control group and the aged rats in the intervention group each had 10 rats. The remaining young and old rats, each 5, after sacrifice, take myocardial nitric oxide synthase Westernblot study. The rats in the control group of young rats and the control rats of the old rats were treated with physiological saline before the experiment, and the rats in the intervention group and the aged rats received the intervention of iNOS blocker-1400W. The isolated hearts of 4 rats were injected with isoproterenol (Iso) for 30 minutes after they were infused with saline and 1400 W respectively and 50% normal coronary flow for 30 minutes. Results: Left ventricular pressure (LVDP) and left ventricular pressure (LVDP) decreased from 13% to 45% in all groups at 30min after myocardial infarction. Iso pumped into the young rats control group and young rats intervention group rats 30min, ventricular function partially recovered, the performance of LVDP and ± dp / dt increased. In contrast, Iso pumped into the aged rat control group, not only failed to improve the ischemic heart failure, but further aggravate cardiac dysfunction. The performance of LVDP and ± dp / dt decreased further by 43% to 60%. Westernblot research