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目的探讨幽门螺杆菌(Hp)感染与胆囊结石形成之间的关系。方法PCR法和快速尿素酶法分别检测胆囊结石患者胆囊黏膜及胆汁Hp DNA和尿素酶,酸滴定法测定胆囊黏膜和胆汁磷脂酶A2(PLA2)活性,并做黏膜和胆汁普通、厌氧和Hp培养。结果黏膜Hp DNA阳性检出率45.24%,胆汁阳性检出率35.71%,其中胆汁Hp DNA检测结果阳性者,其黏膜检测结果均阳性。黏膜Hp DNA阳性组胆汁中PLA2的活性显著高于阴性组胆汁,分别为(480.92±164.21)U/L,(225.01±79.01)U/L;黏膜之间的PLA2活性差异无统计学意义(P>0.05)。黏膜及胆汁尿素酶检测均为阴性,普通、厌氧和Hp培养阴性。结论胆囊结石患者存在Hp感染因素,并可能通过PLA2促进胆囊结石形成。
Objective To investigate the relationship between Helicobacter pylori (Hp) infection and gallstone formation. Methods The gallbladder mucosa and bile Hp DNA and urease were detected by PCR and rapid urease. The activity of gallbladder mucosa and bile phospholipase A2 (PLA2) were measured by acid titration. The mucosa and common bile, anaerobic and Hp to cultivate. Results The positive rate of Hp DNA in mucosa was 45.24%, and the positive rate of bile was 35.71%. The results of mucosal examination of positive Hb DNA were positive. The activity of PLA2 in bile of Hp DNA positive group was significantly higher than that of negative group (480.92 ± 164.21) U / L, (225.01 ± 79.01) U / L, respectively. There was no significant difference in PLA2 activity between mucosa > 0.05). Mucosal and bile urease tests were negative, normal, anaerobic and Hp culture negative. Conclusion There are Hp infection in patients with gallstone and may promote the formation of gallstones by PLA2.