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目的 :研究 D-丝氨酸对大鼠小脑 NMDA受体 /NO/c GMP神经通路的调节作用。方法 :采用清醒大鼠脑微透析技术 ,应用放免方法测定小脑透析液中 c GMP含量。结果 :小脑内局部灌流 NMDA可明显提高细胞外 c GMP水平。NMDA受体通道阻断剂 MK-80 1可明显对抗 NMDA引起的 c GMP反应。单独应用 NMDA对基础的 c GMP水平没有影响。D-丝氨酸可剂量依赖性加强NMDA2 0 0 μmol.L- 1 引起的 c GMP增加作用。单独应用 D-丝氨酸对基础的c GMP水平没有影响。结论 :说明内源性兴奋氨基酸对 NMDA受体激活作用很弱。也说明在小脑 NMDA受体的甘氨酸部位并没有完全饱和 ,应用选择性的甘氨酸部位的激活剂 D-丝氨酸可以调解 NMDA受体复合物的功能。
AIM: To investigate the regulatory effect of D-serine on the NMDA receptor / NO / c GMP neural pathway in rat cerebellum. Methods: Using conscious rat brain microdialysis technique, the content of c GMP in cerebellum dialysate was determined by radioimmunoassay. Results: Local perfusion of cerebellar NMDA significantly increased extracellular c GMP levels. NMDA receptor channel blocker MK-80 1 can obviously antagonize NMDA-induced c-GMP reaction. NMDA alone had no effect on basal c GMP levels. D-serine could enhance the effect of NMDA on the increase of cGMP induced by NMDA 200 μmol·L -1 in a dose-dependent manner. D-serine alone had no effect on basal c GMP levels. Conclusion: It shows that endogenous excitatory amino acids have a weak effect on NMDA receptor activation. Also shows that in the cerebellum NMDA receptor glycine part is not fully saturated, the application of selective glycine site activator D-serine can mediate the function of NMDA receptor complex.