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首次报道了转基因不结球白菜实验地中 ,以除草剂抗性基因bar为标记基因的外源基因流动以及在芸薹属不同栽培作物中的基因渗入情况 .结果表明 ,外源基因能随花粉的散布流动 ,距离转基因花粉源越远 ,流动频率越低 .距离转基因白菜 0～ 3m处为花粉的主要流动区域 ,其频率占总流动率的 5 0 %左右 .2 0m隔离带后基因的流动频率仅为10m隔离带后的 1/2 0 .自然条件下 ,白菜中的外源基因在芸薹属同基因组作物中具有较高的渗入频率 ;在基因组具部分同源性的甘蓝型油菜中具一定的渗入频率 ;不能渗入甘蓝基因组中 .基因的渗入频率与作物间的亲缘性、隔离距离等多因素相关 .探讨了基因流动频率与基因渗入频率的差异 ,提出在有显性标记基因及花粉的供体、受体完全亲合的情况下 ,可以用基因的渗入频率来反应田间的基因流动情况 .图 4表 4参 12
We first reported the exogenous gene flowering in transgenic non-heading Chinese cabbage (Brassica campestris ssp.chinensis) with bar-labeled marker gene and introgression in different cultivated Brassica plants.The results showed that the exogenous gene could be expressed with pollen , The farther away from the source of transgenic pollen, the lower the frequency of flow.The distance from 0 ~ 3m of transgenic cabbage is the main flow area of pollen, its frequency accounts for about 50% of the total flow rate. The frequency is only 1/2 of the isolator after 10 m isolation.Exogenous genes in Chinese cabbage have a higher frequency of infiltration in the same genus Brassica plants under natural conditions.In the Brassica napus And had a certain infiltration frequency; it could not penetrate into the genome of cabbage.The frequency of gene infiltration was related to the inter-crop affinity, isolation distance and other factors.The differences of gene flow frequency and gene infiltration frequency were discussed, Pollen donor, the receptor is completely in the case of affinity, you can use gene penetration frequency to respond to field gene flow situation.