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[目的]探讨上皮膜蛋白(epithelial membrane protejn 1,EMP1)在食管鳞癌中的表达及意义。[方法]采用免疫组织化学、Western blot方法检测64例食管鳞癌癌组织及距其癌组织边缘2cm以上的镜下未见癌浸润的正常组织中EMP1蛋白的表达情况。[结果]免疫组织化学结果表明,EMP1蛋白在食管鳞癌组织表达阳性率明显低于正常食管组织(48.4%vs 82.8%,P<0.05)。Western blot结果表明,EMP1蛋白在食管鳞癌组织的相对表达量较正常食管组织的相对表达量明显降低,二者比较差异具有统计学意义(P<0.05)。EMP1蛋白表达与食管鳞癌患者年龄、性别及肿瘤大小无关(P>0.05);而与有无淋巴结转移、肿瘤临床分期以及细胞分化程度有关(P<0.05)。[结论]食管鳞癌组织中EMP1蛋白表达明显减低,且与食管鳞癌有无淋巴结转移、临床分期及肿瘤细胞分化程度有关。
[Objective] To investigate the expression and significance of epithelial membrane protejn 1 (EMP1) in esophageal squamous cell carcinoma. [Method] Immunohistochemistry and Western blot were used to detect the expression of EMP1 protein in 64 esophageal squamous cell carcinoma tissues and 2cm above the edge of their cancerous tissues. [Results] Immunohistochemistry results showed that the positive expression rate of EMP1 protein in esophageal squamous cell carcinoma tissues was significantly lower than that in normal esophageal tissues (48.4% vs 82.8%, P <0.05). Western blot results showed that the relative expression of EMP1 protein in esophageal squamous cell carcinoma tissue was significantly lower than that in normal esophageal tissue, the difference was statistically significant (P <0.05). EMP1 protein expression was not associated with age, sex and tumor size (P> 0.05), but with or without lymph node metastasis, clinical stage and cell differentiation (P <0.05). [Conclusion] The expression of EMP1 protein in esophageal squamous cell carcinoma is significantly reduced, and it is related to the presence or absence of lymph node metastasis, clinical stage and tumor cell differentiation in esophageal squamous cell carcinoma.