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采用共沉淀法制备了复合转化生长因子-β1(TGF-β1)的磺化壳聚糖/聚赖氨酸纳米粒子(SCS/PLL/TGF-β1),研究了磺化壳聚糖的浓度对纳米粒子粒径和表面电位的影响.所得纳米微粒的粒径在240~290nm、表面电位在-0.4~-1.3 mV之间可调.采用透射电子显微镜表征了磺化壳聚糖/聚赖氨酸(SCS/PLL)和SCS/PLL/TGF-β1纳米粒子的形貌.通过体外骨髓间充质干细胞(BMSCs)培养实验,分别考察了SCS/PLL/TGF-β1纳米粒子对BMSCs的增殖、氨基葡聚糖(GAGs)分泌和Ⅱ型胶原基因表达的影响.结果显示,相对于等量的SCS/PLL或同浓度的自由TGF-β1,SCS/PLL/TGF-β1纳米粒子可有效保护TGF-β1的活性,促进BMSCs向软骨细胞分化,可望应用于软骨修复材料等领域的研究.
The sulfated chitosan / polylysine nanoparticles (SCS / PLL / TGF-β1) with TGF-β1 were prepared by co-precipitation method. The effects of concentration of sulfonated chitosan Nano-particle size and surface potential.The particle size of the obtained nanoparticles at 240 ~ 290nm, the surface potential adjustable between -0.4 ~ -1.3 mV Transmission electron microscopy was characterized by sulfonated chitosan / polylysine (SCS / PLL) and SCS / PLL / TGF-β1 nanoparticles.Experiment of BMSCs culture in vitro, we investigated the effects of SCS / PLL / TGF-β1 nanoparticle on the proliferation, GAGs secretion and type Ⅱ collagen gene expression.The results showed that SCS / PLL / TGF-β1 nanoparticles could effectively protect TGF-β1 cells against TGF-β1 and SCF / -β1 activity, and promote the differentiation of BMSCs to chondrocytes, which is expected to be used in the field of cartilage repair materials and other fields.