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谷氧还蛋白(Glutaredoxin,GRX)是一类小分子氧化还原酶,能够调节蛋白质的氧化还原状态,在逆境胁迫中发挥重要的作用。本研究从美洲商陆鉴定克隆了一个重金属镉诱导表达的谷氧还蛋白基因Pa GRXC13。该基因全长306 bp,编码101个氨基酸。Pa GRXC13蛋白分子量约为11.189 k D,属于典型的CC型谷氧还蛋白。Pa GRXC13与不同来源CC型谷氧还蛋白的氨基酸序列保守性较高,一致率介于67.0~83.5%之间。系统进化分析显示,Pa GRXC13与菠菜(Spinacia oleracea)、中粒咖啡(Coffea canephora)和克莱门柑橘(Citrus clementina)的GRXC13处在同一个进化分支上。该基因在美洲商陆的叶和须根中具高转录水平,在块根和茎中表达量低。
Glutaredoxin (GRX) is a kind of small molecule redox enzyme that regulates the redox state of proteins and plays an important role in the stress of stress. In this study, we isolated a cloned glutathione gene, Pa GRXC13, induced by cadmium. The gene is 306 bp in length and encodes 101 amino acids. Pa GRXC13 protein molecular weight of about 11.189 kD, is a typical CC type glutaredoxin. The amino acid sequence of Pa GRXC13 and different types of CC glutaredoxin were highly conserved, with concordance rates ranging from 67.0% to 83.5%. Phylogenetic analysis showed that Pa GRXC13 was on the same branch of evolution as GRXC13 in Spinacia oleracea, Coffea canephora and Citrus clementina. This gene is highly transcribed in leaves and fibrous roots of Pokeweed, and is low in roots and tubers.