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目的探讨HBV感染患者外周血可溶性B7-H3(sB7-H3)的表达及其临床意义。方法ELISA法检测65例慢性HBV感染患者(CHB组)、42例肝硬化和肝癌患者(LC-HCC组)以及20例健康志愿者(HC组)外周血sB7-H3含量,分析sB7-H3表达与ALT、AST、HBV-DNA水平和调节性T细胞(Treg细胞)比例的相关性。结果 CHB组和LC-HCC组外周血sB7-H3的含量较HC组升高[(3.81±0.12)ng/ml和(5.34±0.27)ng/ml vs.(2.97±0.18)ng/ml](P均<0.01),且LC-HCC组高于CHB组(P<0.01)。CHB组sB7-H3表达与ALT、AST、HBV-DNA水平和Treg细胞比例均呈正相关(r=0.3072、r=0.3659、r=0.2847、r=0.4651,P<0.01),LC-HCC组sB7-H3表达与Treg细胞比例呈正相关(r=0.6037,P<0.01)。结论 HBV感染患者外周血的sB7-H3表达水平升高,且与肝损伤指标、Treg细胞比例呈正相关。
Objective To investigate the expression of soluble B7-H3 (sB7-H3) in peripheral blood of patients with HBV infection and its clinical significance. Methods The levels of sB7-H3 in peripheral blood of 65 patients with chronic HBV infection (CHB group), 42 patients with liver cirrhosis and hepatocellular carcinoma (LC-HCC group) and 20 healthy volunteers (HC group) were detected by ELISA. The expression of sB7-H3 And ALT, AST, HBV-DNA levels and regulatory T cells (Treg cells) ratio. Results The levels of sB7-H3 in peripheral blood of CHB group and LC-HCC group were higher than those of HC group [(3.81 ± 0.12) ng / ml and (5.34 ± 0.27) ng / ml vs. (2.97 ± 0.18) ng / ml] P <0.01), and higher in LC-HCC group than in CHB group (P <0.01). The expression of sB7-H3 in CHB group was positively correlated with the levels of ALT, AST, HBV-DNA and Treg cells (r = 0.3072, r = 0.3659, r = 0.2847, r = 0.4651, The expression of H3 was positively correlated with the proportion of Treg cells (r = 0.6037, P <0.01). Conclusion The expression of sB7-H3 in peripheral blood of patients with HBV infection is increased, and it is positively correlated with the index of liver injury and the proportion of Treg cells.