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目的观察选择性环氧合酶2(COX2)抑制剂NS398对结肠癌细胞系SW480中Stat5与PPARδ信号转导通路的影响,以期初步阐明选择性COX2抑制剂抗结直肠癌非COX2依赖性的作用机制。方法应用RTPCR检测结肠癌细胞系SW480中COX2mRNA表达水平,用选择性COX2抑制剂NS398处理结肠癌细胞系SW480,Western印迹检测Stat5与PPARδ信号转导通路成员表达,四甲基偶氮唑盐(MTT)法检测细胞增殖状态,流式细胞技术检测细胞周期与凋亡情况。结果结肠癌细胞系SW480中未检测到COX2mRNA表达,NS398(75μmol/L)作用于SW480细胞72h后,G1期细胞比率由31.2%上升至40.6%,S期细胞比率由52.8%下降至41.2%,细胞增殖受抑制。Stat5、PPARδ、细胞周期蛋白D1与BclxL表达水平随NS398作用时间延长而下降。结论选择性COX2抑制剂NS398可能通过非COX2依赖途径影响结肠癌细胞的增殖。
Objective To investigate the effects of selective cyclooxygenase 2 (COX2) inhibitor NS398 on Stat5 and PPARδ signal transduction pathways in human colon cancer cell line SW480 in order to initially clarify the non-COX2-dependent effects of selective COX2 inhibitors on colorectal cancer mechanism. Methods The expression of COX2 mRNA in colon cancer cell line SW480 was detected by RTPCR. The colon cancer cell line SW480 was treated with selective COX2 inhibitor NS398. The expression of Stat5 and PPARδ signal transduction pathway was detected by Western blotting. The MTT ) Method to detect cell proliferation status, flow cytometry to detect cell cycle and apoptosis. Results The expression of COX2 mRNA was not detected in SW480 colon cancer cells. After treated with NS398 (75μmol / L) for 72h, the percentage of G1 phase cells increased from 31.2% to 40.6% and the percentage of S phase cells decreased from 52.8% to 41.2% Cell proliferation is inhibited. The expression of Stat5, PPARδ, cyclin D1 and BclxL decreased with the prolongation of NS398 effect. Conclusion The selective COX2 inhibitor NS398 may affect the proliferation of colon cancer cells through a non-COX2-dependent pathway.