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目的：观察人肺癌裸鼠移植瘤整合蛋白表达与其转移的相关性。方法：以ＡＢＣ免疫组化染色，对转移力由低到高依次为ＰＬＡ－８０１Ｃ、Ａｎｉｐ９７３、ＰＬＡ－８０１Ｄ、ＰＬＡ－８０１ＤＬ四株人肺癌裸鼠移植瘤作整合蛋白亚单位表达的观察，并在裸鼠体内观察整合蛋白β１抗体及其配体ＲＧＤ多肽对移植瘤生长及转移的影响。结果：四株移植瘤均呈α２、α５、α６Ｂ阴性。β１的表达除Ａｎｉｐ９７３呈强阳性、ＰＬＡ－８０１ＤＬ呈弱阳性外，也均呈阴性。但四株移植瘤除ＰＬＡ－８０１Ｃ外，其余３株均呈α６Ａ强阳性。β１抗体及ＲＧＤ多肽对ＰＬＡ－８０１ＤＬ移植瘤的转移不见明显影响。结论：４株人肺癌移植瘤总体上呈β１亚族整合蛋白低表达，表达水平与其转移无显著相关。
Objective: To observe the correlation between the expression of integrin and its metastasis in human lung cancer xenografts in nude mice. Methods: Immunohistochemical staining with ABC was used to observe the expression of integrin subunits in lung cancer xenografts from PLA-801C, Anip973, PLA-801D and PLA-801DL. The effects of integrin β1 antibody and its ligand RGD polypeptide on growth and metastasis of transplanted tumors were observed in nude mice. Results: All four tumors were negative for α2, α5 and α6B. The expression of β1 was negative except that Anip973 was strongly positive and PLA-801DL was weakly positive. However, except for PLA-801C, all the other 4 tumors showed strong α6A positive. Β1 antibody and RGD polypeptide had no significant effect on the migration of PLA-801DL xenografts. CONCLUSIONS: Four human lung cancer xenografts exhibit low expression of the integrin β1 subfamily, and their expression levels are not significantly associated with their metastasis.