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采用紫外线照射与药物培养基驯化相结合的方法选育抗药性链霉菌,筛选出4株对腐霉利有显著抗性的突变株(US-1,US-2,US-3和US-4)。US-1和US-3对灰葡萄孢菌的抑菌能力显著高于亲本菌株,US-2和US-4的抑菌能力显著低于亲本菌株。颉颃活性最强的US-1菌株与腐霉利的协同作用表明,7份菌液和3份药液混用后,对灰葡萄孢菌的抑菌圈直径为21.97mm,对离体番茄灰霉病的防效高达77.68%,显著高于菌株单独使用的抑菌效果。
Streptomyces resistant to Streptomyces was selected by a combination of UV irradiation and domestication of the culture medium, and four mutants (US-1, US-2, US-3 and US-4 ). The antibacterial activity of US-1 and US-3 against Botrytis cinerea was significantly higher than that of the parent strain, and the antibacterial activity of US-2 and US-4 was significantly lower than that of the parent strain. The synergistic effect of the most active antagonistic fungus US-1 with desubenamycin showed that the antibacterial circle diameter of Botrytis cinerea was 21.97mm after mixed with 7 parts liquid and 3 parts of liquid, Disease control efficiency up to 77.68%, significantly higher than the antibacterial effect of the strain alone.