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以溴化二甲基二癸基烃铵消毒剂为研究对象,以大肠埃希菌、链球菌、志贺菌和金黄色葡萄球菌为受试菌,通过悬液定量杀菌方法,研究在不同温度(37℃,25℃,4℃)、不同作用时间下,溴化二甲基二癸基烃铵的最佳杀菌条件。结果表明,在没有有机干扰物存在时,当溴化二甲基二癸基烃铵含量为125 mg/L时,其在37℃,25℃,4℃下,与大肠埃希菌、链球菌和金黄色葡萄球菌(107cfu/L)作用1 min,杀菌率可达100%;相同条件下100%杀灭志贺菌的含量为250 mg/L。当作用时间为5 min时,除了在4℃不能100%杀灭志贺菌外,31.25 mg/L消毒剂在试验条件下对受试菌均可达到100%的杀灭。分别以大肠埃希菌和金黄色葡萄球菌为受试菌,研究有机干扰物对溴化二甲基二癸基烃铵杀菌作用的影响。结果发现,当干扰物(牛血清白蛋白)的终含量为3%时,在室温下(25℃)杀灭大肠埃希菌和金黄色葡萄球菌(107cfu/L)的条件分别为62.5 mg/L、125 mg/L作用10 min以上;当干扰物终含量为25%时,相同情况下杀灭受试菌的条件分别为125 mg/L、250 mg/L。研究结果为溴化二甲基二癸基烃铵消毒剂的使用提供了基础资料。
With dimethyl didecyl ammonium bromide disinfectant as research object, Escherichia coli, Streptococcus, Shigella and Staphylococcus aureus as tested bacteria, by quantitative suspension sterilization method to study the different temperature (37 ℃, 25 ℃, 4 ℃), the best sterilization conditions of dimethyl didecyl ammonium bromide under different action time. The results showed that when there was no organic interference, when the content of dimethyl didecyl ammonium bromide was 125 mg / L, it was incubated at 37 ℃, 25 ℃, 4 ℃ with Escherichia coli, Streptococcus And Staphylococcus aureus (107cfu / L) for 1 min, the bactericidal rate was up to 100%; under the same conditions, the content of Shigella killed by 100% was 250 mg / L. When the action time was 5 min, except for the fact that Shigella was not 100% killed at 4 ℃, the 31.25 mg / L disinfectant could kill 100% of the tested bacteria under the test conditions. Escherichia coli and Staphylococcus aureus were used as tested bacteria to study the effect of organic interferences on the bactericidal effect of dimethyl didecyl ammonium bromide. As a result, it was found that the conditions for killing Escherichia coli and Staphylococcus aureus (107 cfu / L) were 62.5 mg / L at room temperature (25 ° C) when the final content of the interferent (bovine serum albumin) L and 125 mg / L for 10 min. When the final concentration of interfering substance was 25%, the conditions of killing test bacteria were 125 mg / L and 250 mg / L under the same conditions respectively. The results provide the basic information for the use of dimethyl didecyl ammonium bromide disinfectant.